The offer is based on the generation of recombinant viruses based on a full-length HIV-1 clone in which the following changes have been produced:
The final goal of these modifications is to generate a collection of viral vectors (target vectors) in which specific genes (gag, pol, env) are deleted. All the target vectors carry the renilla-luciferase gene in the position of nef. The Lac-Z gene has been cloned in the position of the deleted HIV-1 fragment, generating target vectors named LacZPol, LacZPr, LacZRT, LacZGag-Pol and LacZEnv, where the names indicate the gene substituted by the LacZ gene. We have generated the following target vectors: