The offer is based on the generation of recombinant viruses based on a full-length HIV-1 clone in which the following changes have been produced:

• Introduction of specific enzyme-restriction sites along the viral vector.
• Deletion of the sequences corresponding to the nef gene and replacement by the renilla-luciferase gene in this position.
• Replacement of specific viral sequences by the Lac-Z gene.

Introduction of specific enzyme-restriction sites along the viral vector:

Deletion of the sequences corresponding to the nef gene and replacement by the renilla-luciferase gene in this position:

 

Replacement of specific viral sequences by the Lac-Z gene:

The final goal of these modifications is to generate a collection of viral vectors (target vectors) in which specific genes (gag, pol, env) are deleted. All the target vectors carry the renilla-luciferase gene in the position of nef. The Lac-Z gene has been cloned in the position of the deleted HIV-1 fragment, generating target vectors named LacZPol, LacZPr, LacZRT, LacZGag-Pol and LacZEnv, where the names indicate the gene substituted by the LacZ gene. We have generated the following target vectors: